That is because the ISO format and the underlying file systems it uses (ISO9660 or UDF) are designed for optical boot, which is a completely different beast from regular HDD or USB boot. For one thing optical media, and therefore (regular) ISO images, don't have a partition table, which is (usually) essential for HDD or USB boot, and they also (usually) don't have a Master Boot record, a.k.a. MBR, which is essential for BIOS boot.