In order to construct a comprehensive library of allergenic proteins, the curated Allergome database9 was downloaded from UniProt (accessed 6 August 2017) and used as input to the PhIP-Seq pepsyn library design pipeline7. The 1847 proteins of the Allergome database were represented as a set of 19,332 56 amino acid peptide tiles with 28 amino acid overlaps, which were encoded by a library of synthetic 200-mer oligonucleotides (Fig. 1a). This library was amplified and cloned into the T7 phage display system6 for automated serological profiling7. We refer to this library as the T7 AllerScan library.