We observed consistent patterns of wheat reactivity among the allergic cohort; some peptides were reactive in over 80% of individuals with wheat allergy, whereas most peptides did not react with sera from any individual included in this study. Dominant wheat reactivities tended to occur in highly repetitive regions of allergenic proteins; this phenomenon has also been described for other allergens25. Additionally, we compared isotype differences in wheat peptide reactivity and found notable discordance in IgE and IgG reactivity for most peptides, as has been observed in other epitope studies; only 16% of reactive peptides were bound by both IgE and IgG isotypes. It is possible that this discordance may be partially explained by competition between the two antibody classes. Future experiments in which purified IgE and IgG are used for AllerScan analysis will test this hypothesis. Future studies using AllerScan may also provide insight into questions about B cell development, class-switching, and affinity maturation, particularly when used in high-resolution longitudinal studies or in animal models of allergic disease.