Due to dynamic changes in biotechnological systems, none of the three coding schemes represents a suitable solution from the perspective of current DNA sequencer designs: Huffman codes are fixed-to-variable length compressors that can lead to catastrophic error propagation in the presence of sequencing noise; the same is true of differential codes. Homopolymers do not represent a significant source of errors in Illumina sequencing platforms6, while single parity redundancy or RS codes and differential encoding are inadequate for combating error-inducing sequence patterns such as long substrings with high GC content6. As a result, assembly errors are likely and were observed during the readout process described in4.