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There is limited literature on the global prevalence of MS in layers. Some studies reported that MS is found in 73% of layer flocks in the Netherlands [28], 90% in China [29], 40.3% in Portugal [10], 72.7% in Brazil [11], 69% in Australia [16] and 68% in France [18]. However, to our knowledge, no prevalence data for the EAA syndrome (production of eggs with eggshell apex abnormalities in laying-hen flocks) is available.