Unraveling the linkages between FB traits and edaphoclimatic conditions is difficult because: (1) the long period of formation of truffle FBs hinders the identification of causal relationships, (2) FB traits can be mediated in various ways such as fungal physiology, host physiology or spatio-temporal distribution of resources9 and (3) some traits could be linked and trade off with each other. The reproductive investment of black truffle (determined by FB number and size) likely depends on the availability of resources, among which those supplied by the host are fundamental13. Water, temperature and their effect on soil O2 and CO2 diffusion are also key factors for the FB formation in mycorrhizal fungi9. On the other hand, maturity and ripeness play an ecologically relevant role in truffle dispersal, which requires completely functional spores as well as the emission of volatile signals attracting animal dispersal vectors. However, the relationship between spore maturity and aroma development is not well understood yet14. Attracted by truffle volatiles, truffle beetles (genus Leiodes), are among the most frequent consumers of truffle FBs in Spanish T. melanosporum orchards, reaching in some cases high levels of FB infestation15. Truffle beetles persist as diapausant larvae in the orchard soil from May to September, with adults emerging to copulate and lay eggs near a truffle FB from mid-September to April16. One to 1.5 months may be required from the moment adults emerge from the soil until the mycophagous larvae appear15.